Molecular markers for population genetic analysesin the family Psittacidae (Psittaciformes, Aves)

Blue-and-yellow Macaw (Ara ararauna) Science Article 1

abstract

The selection of molecular markers for population studies is an important tool for biodiversity conservation. The familyPsittacidae contains many endangered and vulnerable species and we tested three kinds of molecular markers fortheir potential use in population studies of five psitacid species: 43 hyacinth macaws (Anodorhynchus hyacinthinus),42 blue-and-yellow macaws (Ara ararauna), 23 red-and-green macaws (Ara chloroptera), 19 red-spectacled amazons(Amazona pretrei); and 18 red-tailed amazons (Amazona brasiliensis). We tested 21 clones from a genomic libraryof golden conure (Guarouba guarouba) minisatellites and 12 pairs of microsatellite primers developed for thedomestic chicken (Gallus gallus) and A. hyacinthinus. We also tested seven tetranucleotide repeat primers for theirability to amplify regions between microsatellite loci (inter simple sequence repeats, ISSRs). We were able to selectseven markers that were variable in different degrees for three species (A. hyacinthinus, A. chloroptera and A.ararauna). The mini and microsatellites produced more polymorphic patterns than the ISSRs. The genetic variabilityof the species studied seems to be correlated with their endangered status.

Patricia J. Faria and Cristina Y. Miyaki, Genetics and Molecular Biology, 29, 2, 231-240 (2006)

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